公山羊β防御素104a生物信息学分析及表达特性研究

doi:10.11843/j.issn.0366-6964.2015.02.007

Abstract

Abstract:

The objectives of this study were to analyze the goat beta-defensins 104a (gBD104a) gene by bioinformatics，and to investigate the expression levels of gBD104a in various tissues of adult bucks and the location in reproduction organ and sperms.Bioinformatics analysis of gBD104a (GenBank No.：KJ508074.1) was made by software online.Relative mRNA expressions were detected by real-time fluorescent quantitative PCR.Cellular localization of gBD104a in testes，epididymidis and sperms were examined by immunohistochemistry.The results showed that：(1) The results of bioinformatics analysis showed that the cDNA of gBD104a gene contains 324 nucleotides，including a complete open reading frame (ORF) encoding 107 amino acids，software online predictded 1-19 amino acids as signal peptide area，27-55 amino acids for potential beta defensins motif，7 potential O-glycosylation sites on C-terminal；(2) The results of QRT-PCR showed that gBD104a mRNA was widely expressed，but the relative expression of gBD104a mRNA was the highest in cauda epididymis.Expressions of gBD104a mRNA in caput epididymis，tracheal，abomasums，jejunum and ileum were more than those in other tissues；(3)The results of immunohistochemistry showed that there were strong positive signal of gBD104a in pseudostratified ciliated columnar epithelium of caput and corpus，and strong positive signal in columnar epithelium of cauda.gBD104a was located on the sperm acrosome and mitochondria surface.gBD104a was an epididymal secretory glycoprotein.gBD104a mRNA was widely expressed in various tissues of bucks，while the highest expression levels was in cauda epididymis.gBD104a was coated on sperm surface，which maybe play an important role in sperm motility，acrosome reaction and capacitation process.Therefore，the physiological functions of gBD104a needs be further researched.

CLC Number:

S827.2

REN You-she，ZHANG Guo-lin，GUO Li-na,ZHANG Chun-xiang,ZHENG Ya-lin，ZHANG Cai-xia,QIAO Li-ying，JIN Li，Lü Li-hua，LIU Wen-zhong. Bioinformatics Analysis and Expression Characteristics of Goat Beta-defensin 104a Gene in Adult Bucks[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(2): 219-227.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2015.02.007

https://www.xmsyxb.com/EN/Y2015/V46/I2/219

References

［1］GANZ T.Defensins：antimicrobial peptides of innate immunity［J］.Nat Rev Immunol，2003，3：710-720.
［2］LEHRER R I.Primate defensins ［J］.Nat Rev Microbiol，2004，2：727-738.
［3］DIAMOND G，BEVINS C L.β-Defensins：endogenous antibiotics of the innate host defense response ［J］.Clin Immunol Immunopathol，1998，88：221-225.
［4］SEMPLE F，DORIN J R.Beta-defensins：multifunctional modulators of infection，inflammation and more ［J］.Innate Immunol，2012，4:337-348.
［5］OH J，LEE J，WOO J M，et al.Systematic identification and integrative analysis of novel genes expressed specifically or predominantly in mouse epididymis ［J］.BMC Genomics，2006，7：314.
［6］PATIL A A，CAI Y，SANG Y，et al.Cross-species analysis of the mammalian beta-defensin gene family：presence of syntenic gene clusters and preferential expression in the male reproductive tract ［J］.Physiol Genomics，2005，23：5-17.
［7］ZHOU Y S，WEBB S，LETTICE L，et al.Partial deletion of chromosome 8 beta-defensin custer confers sperm dysfunction and infertility in male mice ［J］.PLoS Genet, 2013，9(10)：e1003826.
［8］HEGUO Y，JING D，YIHUA G，et al.The novel human β-Defensin 114 regulates lipopolysaccharide (LPS)-mediated inflammation and protects sperm from motility loss ［J］.J Biol Chem，2013，288(17)：12270-12282.
［9］SURESH Y，KATHERINE G H，FRANK S F，et al.The androgen-regulated epididymal sperm-bingding protein，human β-defensin 118(DEFB118) (formerly ESC42)，is an antimicrobial β-defensin ［J］.Endocrinology，2004，145(7)：3165-3173.
［10］RODRIJUEZ-JIMEEZ F J，KRAUSE A，SCHULZ S，et al.Distribution of new human β-defensin genes clustered on chromosome 20 in functionally different segments of epididymis ［J］.Genomics，2003，81：175-183.
［11］TOLLNER T L，YUDIN A I，TREECE C A，et al.Macaque sperm release ESP13.2 and PSP94 during capacitation：the absence of ESP13.2 is linked to sperm-zona recognition and binding ［J］.Mol Reprod Dev，2004，69：325-327.
［12］THEODORE L，TOLLNER，YUDIN A I，et al.Macaque sperm coating protein DEFB126 facilitates sperm penetration of cervical mucus ［J］.Hum Reprod，2008，23(11)：2523-2534.
［13］YUDIN A I，TOLLNER T L，TREECE C A，et al.β-defensin 22 is a major component of the mouse sperm glycocalyx ［J］.Reproduction，2008，136(6)：753-765.
［14］PEDRO C C，MARIANO G B，FABIAN B，et al.A role for the chemokine receptor CCR6 in mammalian sperm motility and chemotaxis ［J］.Cell Physiol，2014，229(1)：24418-24441.
［15］施力光，荀文娟，岳文斌，等.山羊PHGPx基因在不同组织中的表达特点及性腺中的定位［J］.中国农业科学，2010，43(17)：3653-3659.
SHI G L，XUN W J，YUE W B，et al.Tissue expression profile and cellular localization of PHGPx in gonad of goat［J］.Scientia Agricultura Sinica，2010，43(17)：3653-3659.(in Chinese)
［16］MARIA C W A，LUCIANA H，KATHERINE G H，et al.Novel aspects of the sperm-associated antigen 11 (SPAG11) gene organization and expression in cattle (Bos taurus) ［J］. Biol Reprod，2007，76：1103-1116.
［17］刘凌云，薛绍白，柳惠图.细胞生物学［M］.北京：高等教育出版社，2002：89-92.
LIU L Y，XUE S B，LIU H T.Cell biology［M］.Beijing：Higher Education Press，2002：89-92.(in Chinese)
［18］SCHROTER S，OSTERHOFF C，MCARDLE W.The glycocalyx of the sperm surface ［J］.Hum Reprod Update，1999，5：302-313.
［19］TOLLNER T L，YUDIN A I，TARANTAL A F，et al.Beta-defensin 126 on the surface of macaque sperm mediates attachment of sperm to oviductal epithelia ［J］.Biol Reprod，2008，78：400-412.
［20］HINTON B T，PALLADINO M A，RUDOLPH D，et al.The role of the epididymis in the protection of spermatozoa ［J］.Current Topics Dev Biol, 1996，33：61-102.
［21］JONES R C.To store or mature spermatozoa? The primary role of the epididymis ［J］. Int J Androl，1999，22：57-67.
［22］HINTON B T，PALLADINO M A.Epididymal epithelium：its contribution to the formation of a luminal fluid microenvironment ［J］.Microscopy Res Tech，1995，30：67-81.
［23］MOORE H D.Contribution of epididymal factors to sperm maturation and storage ［J］.Andrologia，1998，30：233-239.
［24］DACHEUX J L，GATTI J L，DACHEUX F.Contribution of epididymal secretory proteins for spermatozoa maturation ［J］.Microscopy Res Tech, 2003，61：7-17.
［25］SCOTT A J，TERRY T T，HYUN J B，et al.The rat epididymal transcriptome：comparison of Segmental gene expression in the rat and mouse epididymides ［J］.Biol Reprod，2007，76：561-570.
［26］XIN A，ZHAOY，YU H，et al.Soluble fusion expression，characterization and localization of human beta-defensin 6［J］. Mol Med Rep，2014，9(1)：149-155.
［27］TOSHIMORE K，ARAKI S，OURA C，et al.Loss of sperm surface sialic acid induces phagocytosis：anassay with a monoclonal antibody T21，which recognizes a 54K sialoglycoprotein ［J］.Archives Androl, 1991，27：79-86.

[1]	YAN Xiaochun, XI Haijiao, LI Jinquan, WANG Zhiying, SU Rui. Study on Estimates of Genomic Breeding Value of Fleece Traits in Inner Mongolia Cashmere Goats [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 120-128.
[2]	ZHANG Chenjian, LI Yinxia, DING Qiang, LIU Weijia, WANG Huili, HE Nan, WU Jiashun, CAO Shaoxian. Efficient Preparation of CRISPR/Cas9-mediated Goat SOCS2 Gene Edited Embryos [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 129-141.
[3]	MENG Qiuchi, LU Guangyu, CHEN Dingshuang, LIN Yaqiu, WANG Ruilong, ZHONG Chaosong, WANG Yong, LIU Wei, WANG Youli, LI Yanyan, LI Zhixiong. Goats GPR35 Gene Expression Characteristics Analysis and the Effect on Subcutaneous Fat Cell Differentiation [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 4993-5007.
[4]	HE Zhicheng, QIN Xiaochen, Lü Yongqiang, LI Xiujin, BIAN Huilong, LUO Jun, LI Cong. Multiple Stepwise Regression Analysis and Growth Curve Fitting of Body Measurement Traits in Saanen Dairy Goats [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5301-5311.
[5]	ZHANG Renbao, ZHOU Donghui, ZHOU Lisheng, GAO Xiaoxiao, LIU Nan, HE Jianning. Analysis of Genetic Structure of Conservation Population in Jining Gray Goats Based on 70 K SNP Chip [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2836-2847.
[6]	SHAO Yuexin, ZHANG Xinyu, GE Liyan, SHI Huaiping. Cloning and RNA Interference Analysis of ATF4 Gene in Xinong Saanen Dairy Goat [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2353-2364.
[7]	XU Tiantian, ZHANG Tongtong, WANG Meng, WANG Xin. Transcription Factor Foxq1 Affects the Proliferation of Hair Follicle Stem Cells in Cashmere Goats via WNT/β-catenin Signaling Pathway [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2653-2661.
[8]	QUBI Wuqie, LI Yanyan, LI Xin, WANG Yong, WANG Youli, LIU Wei, ZHU Jiangjiang, LIN Yaqiu. APOA4 Gene Inhibits Intramuscular Adipocyte Differentiation in Goat [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 1927-1938.
[9]	MA Keyan, HAN Jintao, BAI Yaqin, LI Taotao, MA Youji. Genetic Diversity Analysis of Yongdeng Qishan Sheep Based on Specific-Locus Amplified Fragment Sequencing [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 1939-1950.
[10]	ZHU Junru, WEI Wei, PEI Dangshuai, ZHANG Fenque, DUAN Yu, GUO Yongfeng, JIANG Yue, XIA Shuli, HAN Jing, HOU Jinxing, AN Xiaopeng. Effects of PDCD4 on the Apoptosis of Dairy Goat Mammary Epithelial Cells and the Synthesis of β-casein and TG [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1429-1440.
[11]	CHEN Cancan, JIANG Jing, SUN Xiaoyan, REN Hangxing, LI Jie. Expression of AGRP Gene in Goat Tissue and Its Action Mechanism on Melanin Production [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1441-1451.
[12]	YE Junning, DENG Ming, XUE Huiwen, LIU Guangbin, ZOU Xian, SUN Baoli, GUO Yongqing, LIU Dewu, LI Yaokun. Identification and Analysis of mRNA and lncRNA Affecting Goat Fetal Muscle Development [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 989-1002.
[13]	LI Xiaobo, LIU Zhanfa, LIU Yue, CHEN Qian, MA Yuehui, ZHAO Qianjun, YE Shaohui. Mining Genes Related to Wool Bending of Zhongwei Goat Based on WGCNA and GSEA [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 2930-2943.
[14]	SUN Hao, ZHE Xiaoshu, ZHANG Wenqi, HAO Fei, LI Wennan, LIU Jie, LIU Dongjun. Effects of Chaetocin on Histone Methylation Modification of Cashmere Goats ADSCs [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(7): 2380-2389.
[15]	LIU Kehan, WANG Yong, LI Yanyan, WANG Chongyang, ZHU Jiangjiang, XIONG Yan, LI An, LIN Yaqiu. Effects of SRSF10 on the Differentiation of Intramuscular Preadipocytes in Goats [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(6): 1768-1778.

Bioinformatics Analysis and Expression Characteristics of Goat Beta-defensin 104a Gene in Adult Bucks

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments